Publications

2026+

• Fallon, G.; Muraro, S. P.; Ailsworth, S.; Hui, S.; Malonis, R. J.; Tse, A. L.; Miller, E. H.; Chandran, K.; Fremont, D. H.; Diamond, M. S.; Lai, J. R.* Protective Human Antibodies against Powassan Virus. J. Virol., 2026, 100, e0210525. PubMed

2021 - 2025

• Ramos, K. E.; Mariano, M. C.; Mittler, E.; Pardo, R.; Zylberman, V.; Guardado-Calvo, P.; Chandran, K.; Lai, J. R.* Hantavirus GnH Nanoparticle Immunogen Elicits Cross-Neutralizing Antibody Response in Mice. ACS Infect. Dis., 2025, 11, 3061-3070. PubMed

• Mariano, M. C.; Hvasta, M. G.; Dowd, K. A.; Huang, W. C.; Jung, H. S.; Evanson, L. R.; Georgiev, G. I.; Frei, J. C.; Lovell, J. F.; Pierson, T. C.; Kuhlman, B.; Lai, J. R.* Design of Hyperglycosylated Zika virus E Proteins that Focus Antibody Recognition on the Complex E Dimer Epitope. ACS Chem. Biol., 2025, 20, 2105-2119. PubMed

• Ramos, K. E.; Nandigrami, P.; Najafian, A.; Lai, J. R.*; Fiser, A.* Optimization of Sarbecovirus Llama Nanobody Interface via a Combined Computational and Phage Display Protein Engineering Approach. Proc. Natl. Acad. Sci. USA, 2025, 122, e2426438122. PubMed

• Jung, H. S.; Bikash, C. R.; Tao, K.; Lai, J. R.* One-Pot Expression and Assembly of Resurfaced Zika virus E Domain III (rsZDIII) Nanoparticle Immunogens in Mammalian Cells. ACS Omega, 2025, 10, 25068. PubMed

• Wang, A.; Monticelli, S. R.; Wirchnianski, A. S.; Abelson, D. M.; Kuehne, A. I.; Bakken, R. R.; Middlecamp, M.; Weingart, M.; Vergnolle, O.; Bornholdt, Z. A.; Moyer, C. L.; Berrigan, J. L.; West, B. R.; Fels, J. M.; Zeitlin, L.; Herbert, A. S.; Chandran, K.; Bikash, C. R.*; Lai, J. R.* Avidity and Variable Domain Spacing Strongly Influence the Therapeutic Potency of Bispecific Antibodies against Crimean-Congo Hemorrhagic Fever Virus. mBio, 2025, 16, e0320224. PubMed

• Mariano, M.; Jung, H.S.; Vergnolle, O.; Haskell, K.; Evanson, L.; Quevedo, G.; Frei, J. C.; Tong, K.; Thackray, L.; Diamond, M. S.; Lai, J. R.* A Two-Component Cocktail of Engineered DIII Nanoparticles Elicits Broadly Neutralizing Antibody Responses against Dengue virus in Mice. iScience, 2025, 28, 112534. PubMed

• Tong, K.; Hernandez, E. M.; Basore, K.; Fremont, D. H.; Lai, J. R.* Chikungunya Virus E2 B Domain Nanoparticle Immunogen Elicits Homotypic Neutralizing Antibody Response in Mice. Vaccine, 2024, 42, r010854. PubMed

• Ramos KE, Okba NMA, Tan J, Bandawane P, Meade PS, Loganathan M, Francis B, Shulenin S, Holtsberg FW, Aman MJ, McMahon M, Krammer F, Lai JR. Broadly protective bispecific antibodies that simultaneously target influenza virus hemagglutinin and neuraminidase. mBio., 2024, 15, e0108524. PubMed

• Kreutzer AG, Malonis RJ, Parrocha CMT, Tong K, Guaglianone G, Nguyen JT, Diab MN, Lai JR, Nowick JS. Generation and Study of Antibodies against Two Triangular Trimers Derived from Aβ. Pept Sci (Hoboken)., 2024, 116, e24333. PubMed

• Wirchnianski AS, Nyakatura EK, Herbert AS, Kuehne AI, Abbasi SA, Florez C, Storm N, McKay LGA, Dailey L, Kuang E, Abelson DM, Wec AZ, Chakraborti S, Holtsberg FW, Shulenin S, Bornholdt ZA, Aman MJ, Honko AN, Griffiths A, Dye JM, Chandran K, Lai JR. Design and characterization of protective pan-ebolavirus and pan-filovirus bispecific antibodies. PLoS Pathog., 2024, 20, e1012134. PubMed

• Yin P, Davenport BJ, Wan JJ, Kim AS, Diamond MS, Ware BC, Tong K, Couderc T, Lecuit M, Lai JR, Morrison TE, Kielian M. Chikungunya virus cell-to-cell transmission is mediated by intercellular extensions in vitro and in vivo. Nat Microbiol. 2023, 8, 1653-1667. PubMed

• Kreutzer AG, Guaglianone G, Yoo S, Parrocha CMT, Ruttenberg SM, Malonis RJ, Tong K, Lin YF, Nguyen JT, Howitz WJ, Diab MN, Hamza IL, Lai JR, Wysocki VH, Nowick JS. Probing differences among Aβ oligomers with two triangular trimers derived from Aβ. Proc Natl Acad Sci USA. 2023, 22, e2219216120. PubMed

• Malonis, R. J.; Georgiev, G. I.; Haslwanter, D.; VanBlargan, L. A.; Fallon, G.; Vergnolle, O.; Cahill, S. M.; Harris, R.; Cowburn, D.; Chandran, K.; Diamond, M. S.; Lai, J. R.* A Powassan virus Domain III Nanoparticle Immunogen Elicits Neutralizing and Protective Antibodies in Mice. PLoS Path., 2022, 18, e1010573. PubMed

• Georgiev, G. I.; Malonis, R. J.;Wirchnianski, A. S.; Wessel, A. W.; Jung, H. S.; Cahill, S. M.; Nyakatura, E. K.; Vergnolle, O.; Cowburn, D.; Diamond, M. S.; Lai, J. R.* Resurfaced Zika Virus EDIII Nanoparticle Immunogens Elicit Neutralizing and Protective Responses In Vivo. Cell Chem. Biol., 2022, 29, 811-823. PubMed

• Lasso, G.; Khan, S.; Allen, S. A.; Mariano, M.; Florez, C.; Orner, E. P.; Quiroz, J. A.; Quevedo, G.; Massimi, A.; Hegde, A.; Wirchnianski, A. S.; Bortz, R. H. 3rd ; Malonis, R. J.; Georgiev, G. I.;Tong, K.; Herrera, N. G.; Morano, N. C.; Garforth, S. J.; Malaviya, A.; Khokhar, A.; Laudermilch, E.; Dieterle, M. E.; Fels, J. M.; Haslwanter, D.; Jangra, R. K.; Barnhill, J.; Almo, S. C.; Chandran, K.*, Lai, J. R.*; Kelly, L.*; Daily, J. P.*; Vergnolle, O.* Longitudinally Monitored Immune Biomarkers Predict the Timing of COVID-19 Outcomes. PLoS Comp. Biol., 2022, 18, e1009778. PubMed

• Ortigoza, M. B.; Yoon, H.; Goldfeld, K. S.; Troxel, A. B.; Daily, J. P.; Wu, Y.; Li, Y.; Wu, D., Cobb, G. F.; Baptiste, G.; O'Keeffe, M.; Corpuz, M. O.; Ostrosky-Zeichner, L., Amin, A.; Zacharioudakis, I. M.; Jayaweera, D. T.; Wu, Y.; Philley, J. V.; Devine, M. S.; Desruisseaux, M. S.; Santin, A. D.; Anjan, S.; Mathew, R.; Patel, B.; Nigo, M.; Upadhyay, R.; Kupferman, T.; Dentino, A. N.; Nanchal, R.; Merlo, C. A.; Hager, D. N.; Chandran, K.; Lai, J. R.; Rivera, J.; Bikash, C. R.; Lasso, G.; Hilbert, T. P.; Paroder, M.; Asencio, A. A.; Liu, M.; Petkova, E.; Bragat, A.; Shaker, R.; McPherson, D. D.; Sacco, R. L.; Keller, M. J.; Grudzen, C. R.; Hochman, J. S.; Pirofski, L. A; CONTAIN COVID-19 Consortium for the CONTAIN COVID-19 Study Group, Parameswaran, L.; Corcoran, A. T.; Rohatgi, A.; Wronska, M. W.; Wu, X.; Srinivasan, R.: Deng, F. M.; Filardo, T. D.; Pendse, J.; Blaser, S. B.; Whyte, O.; Gallagher, J. M.; Thomas, O. E.; Ramos, D.; Sturm-Reganato, C. L.; Fong, C. C.; Daus, I. M.; Payoen, A. G.; Chiofolo, J. T.; Friedman, M. T.; Wu, D. W.; Jacobson, J. L.; Schneider, J. G.; Sarwar, U. N., Wang, H. E.; Huebinger, R. M.; Dronavalli, G.; Bai, Y.; Grimes, C. Z.; Eldin, K. W.; Umana, V. E.; Martin, J. G.; Heath, T. R.; Bello, F. O.; Ransford, D. L.; Laurent-Rolle, M.; Shenoi, S. V.; Akide-Ndunge, O. B.; Thapa, B.; Peterson, J. L.; Knauf, K.; Patel, S. U.; Cheney, L. L.; Tormey, C. A.; Hendrickson, J. E. Efficacy and Safety of COVID-19 Convalescent Plasma in Hospitalized Patients: A Randomized Clinical Trial. JAMA Int. Med., 2022, 182, 115-126. PubMed

• Wirchnianski, A. S.; Wec, A. Z.; Nyakatura, E. K.; Herbert, A. S.; Slough, M. M.; Kuehne, A. I.; Mittler, E.; Jangra, R. K.; Teruya, J.; Dye, J. M.; Lai, J. R.*, Chandran, K.* Two Distinct Lysosomal Targeting Strategies Afford Trojan Horse Antibodies With Pan-Filovirus Activity. Front. Immunol., 2021, 12, 729851. PubMed

• Ishida, E.; Corrigan, D. T.; Malonis, R. J.; Hofmann, D.; Chen, T.; Amin, A. G.; Chatterjee, D.; Joe, M.; Lowary, T. L.; Lai, J. R.; Achkar, J. M. Monoclonal antibodies from humans with Mycobacterium tuberculosis Exposure or Latent Infection Recognize Distinct Arabinomannan Epitopes. Commun. Biol., 2021, 4, 1181. PubMed

• Haslwanter, D.; Dieterle, M. E.; Wec, A. Z.; O’Brien, C.; Sakharkar, M.; Florez, C.; Tong, K.; Rappazzo, C. G.; Lasso, G.; Vergnolle, O; Wirchnianski, A. S.; Bortz, R. H.; Laudermilch, E.; Fels, J. M.; Mengotto, A.; Malonis, R. J.; Georgiev, G. I.; Quiroz, J.; Wrapp, D,; Wang, N.; Dye, K.; Ranhill, J.; Dye, J. M.; McLellan, J. M.; Daily, J. P.; Lai, J. R., Herbert, A. S.; Walker, L.; Chandran, K.*; Jangra, R. K. A Combination of RBD and NTD Neutralizing Antibodies Limits the Generation of SARS-CoV-2 Spike Neutralization-Escape Mutants. mBio, 2021, 12, e0247321. PubMed

• Malonis, R. J.; Earnest, J. T.; Kim, A. S.; Angeliadis, M.; Holtsberg, F. W.; Aman, M. J.; Jangra, R. K.; Chandran, K.; Daily, J. P.; Diamond, M. S.; Kielian, M.; Lai, J. R.* Near-Germline Human Moncolonal Antibodies Neutralize and Protect against Multiple Arthritogenic Alphaviruses. Proc. Natl. Acad. Sci. USA, 2021, 118, e2100104118. PubMed

• Kim, A. S.; Kafai, N. M.; Winkler, E. S.; Gilliland, T. C. Jr; Cottle, E. L.; Earnest, J. T.; Jethva, P. N.; Kaplonek, P.; Shah, A. P.; Fong, R. H.; Davidson, E.; Malonis, R. J.; Quiroz, J. A.; Williamson, L. E.; Vang, L.; Mack, M.; Crowe, J. E. Jr; Doranz, B. J.; Lai, J. R.; Alter, G.; Gross, M. L.; Klimstra, W. B.; Fremont, D. H., Diamond, M. S.* Pan-protective Anti-alphavirus Human Antibodies Target a Conserved E1 Protein Epitope. Cell, 2021, 184, 4414-4429. PubMed

• Fels, J. M.; Maurer, D. P.; Herbert, A. S.; Wirchnianski, A. S.; Vergnolle, O.; Cross, R. W.; Abelson, D. M.; Moyer, C. L.; Mishra, A. K.; Aguilan, J. T.; Kuehne, A. I.; Pauli, N. T.; Bakken, R. R.; Nyakatura, E. K.; Hellert, J.; Quevedo, G.; Lobel, L.; Balinandi, S.; Lutwama, J. J.; Zeitlin, L.; Geisbert, T. W.; Rey, F. A.; Sidoli, S.; McLellan, J. S.; Lai, J. R., Bornholdt, Z. A.*, Dye, J. M.*, Walker, L. M.*, Chandran, K*. Protective Neutralizing Antibodies from Human Survivors of Crimean-Congo Hemorrhagic Fever. Cell, 2021, 184, 3486-3501. PubMed

• Bortz, R. H. 3rd; Florez, C.; Laudermilch, E.; Wirchnianski, A. S.; Lasso, G.; Malonis, R. J.; Georgiev, G. I.;Vergnolle, O.; Herrera, N. G.; Morano, N. C.; Campbell, S. T.; Orner, E. P.; Mengotto, A.; Dieterle, M. E.; Fels, J. M.; Haslwanter, D.; Jangra, R. K.; Celikgil, A.; Kimmel, D.; Lee, J. H.; Mariano, M. C.; Nakouzi, A.; Quiroz, J.; Rivera, J.; Szymczak, W. A.; Tong, K.; Barnhill, J.; Forsell, M. N. E.; Ahlm, C.; Stein, D. T.; Pirofski, L. A.; Goldstein, D. Y.; Garforth, S. J.; Almo, S. C.; Daily, J. P.; Prystowsky, M. B.; Faix, J. D.; Fox, A. S.; Weiss, L. M.*; Lai, J. R.*; Chandran, K.* Single-Dilution COVID-19 Antibody Test with Qualitative and Quantitative Readouts. mSphere, 2021, 6, e00224-21. PubMed

• Forest, S. K.; Orner, E. P.; Goldstein, D. Y.; Wirchnianski, A. S.; Bortz, R. H.; Laudermilch, E.; Florez, C.; Malonis, R. J.; Georgiev, G. I.; Vergnolle, O.; Lo, Y.; Campbell, S. T.; Barnhill, J.; Cadoff, E. M.; Lai, J. R.; Chandran, K.; Weiss, L. M.; Fox, A. S.; Prystowsky, M. B.; Wolgast, L. R.* Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Qualitative Immunoglobulin G Assays: The Value of Numeric Reporting. Arch. Pathol. Lab. Med., 2021, 145, 929-936. PubMed

• Remmel, J. L.; Frei, J. C.; Butler, S. E.; Lai, J. R., Ackerman, M. E.* Diverse Contributions of Avidity to the Broad Neutralization of Dengue Virus by Antibodies Targeting the E Dimer Epitope. Virology, 2021, 559 57-64. PubMed

• Yoon, H. A.; Bartash, R.; Gendlina, I.; Rivera, J.; Nakouzi, A.; Bortz 3rd , R. H.; Wirchnianski, A. S.; Paroder, M.; Fehn, K.; Serrano-Rahman, L.; Babb, R.; Sarwar, U. N.; Haslwanter, D.; Laudermilch, E.: Florez, C.; Dieterle, M. E.; Jangra, R. K.; Fels, J. M.; Tong, K.; Mariano, M. C.; Vergnolle, O.; Georgiev, G. I.; Herrera, N. G.; Malonis, R. J.; Quiroz, J. A.; Morano, N .C.; Krause, G. J.; Sweeney, J. M.; Cowman, K.; Allen, S. A.; Annam, J.; Applebaum, A.; Barboto, D.; Khokhar, A.; Lally, B. J.; Lee, A.; Lee, M.; Malaviya, A.; Sample, R.; Yang, X. A.; Li, Y.; Ruiz, R. E.; Thota, R.; Barnhill, J.; Goldstein, D. Y.; Uehlinger, J.; Garforth, S. J.; Almo, S. C.; Lai, J. R.; Reyes Gil, M.; Fox, A. S.; Chandran, K.; Wang, T.; Daily, J. P.; Pirofski, L. A. Treatment of Severe COVID-19 with Convalescent Plasma in Bronx, NYC. JCI Insight, 2021, Jan 21: 142270 Online ahead of print. PubMed

2016 - 2020

• Dieterle, M. E.; Haslwanter, D.; Bortz 3rd, R. H.; Wirchnianski, A. S.; Lasso, G.; Vergnolle, O.; Abbasi, S. A.; Fels, J. M.; Laudermilch, E.; Florez, C.; Mengotto, A.; Kimmel, D.; Malonis, R. J.; Georgiev, G.; Quiroz, J.; Barnhill, J.; Pirofski, L. A.; Daily, J. P.; Dye, J .M.; Lai, J. R.; Herbert, A. S.; Chandran, K.*; Jangra, R. K.* A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition. Cell Host Microbe, 2020, 28, 486-496. PubMed

• Herrera, N. G.; Morano, N. C.; Celikgil, A.; Georgiev, G. I.; Malonis, R. J.; Lee, J. H.; Tong, K; Vergnolle, O.; Massimi, A. B.; Yen, L. Y.; Noble, A. J.; Kopylov, M.; Bonanno, J. B.; Garrett-Thomson, S. C.; Hayes, D. B.; Bortz, R. H. 3rd ; Wirchnianski, A. S.; Florez, C.; Laudermilch, E.; Haslwanter, D.; Fels, J. M.; Dieterle, M. E.; Jangra, R. K.; Barnhill, J.; Mengotto, A.; Kimmel, D.; Daily, J. P.; Pirofski, L. A.; Chandran, K.; Brenowitz, M.; Garforth, S. J.; Eng, E. T.; Lai, J. R.*; Almo, S. C.* Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis. ACS Omega, 2020, 6, 85-102. PubMed

• Remmel, J. L.; Beauchemin, K. S.; Mishra, A. K.; Frei, J. C.; Lai, J. R.; Bailey-Kellogg, C.; Ackerman, M. E. Combinatorial Resurfacing of Dengue Envelope Protein Domain III Antigens Selectively Ablates Epitopes Associated with Serotype-Specific or Infection-Enhancing Antibody Responses. ACS Comb. Sci., 2020, 22, 446-456. PubMed

• Malonis, R. J.; Lai, J. R.*; Vergnolle, O. Peptide-Based Vaccines: Current Progress and Future Challenges. Chem. Rev., 2020, 120, 3210-3229. PubMed

• Liu, N.; Girvin, M. E.; Brenowitz, M.; Lai, J. R.* Conformational and Lipid Bilayer-Perturbing Properties of Marburg virus GP2 Segments Containing the Fusion Loop and Membrane-Proximal External Region/Transmembrane Segments. Heliyon, 2019, 5, e03018. PubMed

• Quiroz, J. A.; Malonis, R. J.; Thackray, L. B.; Cohen, C. A.; Pallesen, J.; Jangra, R. K.; Brown, R. S.; Hofmann, D.; Holtsberg, F. W.; Shulenin, S.; Nyakatura, E. K.; Rayannavar, V.; Daily, J. P.; Ward, A. B.; Aman, M. J.; Dye, J. M.; Chandran, K.; Diamond, M. S.; Kielian, M.; Lai, J. R.* Isolation and Characterization of Neutralizing Human Monoclonal Antibodies against Chikungunya Virus that Protect against Disease. PLoS Pathog., 2019, 15, e1008061. PubMed

• Dai, Z.; Lai, J. R.* Isolation of Synthetic Antibodies Against BCL2-Associated X Protein (BAX). Methods Mol. Biol., 2019, 1877, 351-357. PubMed

• Jangra, R. K.; Herbert, A. S.; Li, R.; Jae, L. T.; Kleinfelter, L. M.; Slough, M. M.; Barker, S. L.; Guardado-Calvo, P.; Román-Sosa, G.; Dieterle, M. E.; Kuehne, A. I.; Muena, N. A.; Wirchnianski, A. S.; Nyakatura, E. K.; Fels, J. M.; Ng, M; Mittler, E.; Pan, J.; Bharrhan, S.; Wec, A. Z.; Lai, J. R., Sidhu, S. S.; Tischler, N. D.; Rey, F. A.; Moffat, J.; Brummelkamp, T. R.*; Wang, Z*; Dye, J. M.*; Chandran, K.* Protocadherin-1 Is Essential for Cell Entry by New-World hantaviruses. Nature, 2019, 563, 559-563. PubMed

• Saphire, E. O.*; Schendel, S. L.; Fusco, M. L.; Gangavarapu, K.: Gunn, B. M.; Wec, A. Z.; Halfmann, P. J.; Brannan, J. M.; Herbert, A. S.; Qiu, X.; Wagh, K.; He, S.; Giorgi, E. E.; Theiler, J.; Pommert, K. B. J.; Krause, T. B.; Turner, H. L.; Murin, C. D.; Pallesen, J.; Davidson, E.; Ahmed, R.; Aman, M. J.; Bukreyev, A.; Burton, D. R.; Crowe, J. E. Jr; Davis, C. W.; Georgiou, G.; Krammer, F.; Kyratsous. C. A.; Lai, J. R.; Nykiforuk, C.; Pauly, M. H.; Rijal, P.; Takada, A.; Townsend, A. R.; Volchkov, V.; Walker, L. M.; Wang, C. I.; Zeitlin, L.; Doranz, B. J.; Ward, A. B.; Korber, B.; Kobinger, G. P.*; Andersen, K. G.*; Kawaoka, Y.*; Alter, G.*; Chandran, K.*; Dye, J. M.*; Viral Hemorrhagic Fever Immunotherapeutic Consortium. Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection. Cell, 2018, 174, 938-952. PubMed

• Gunn, B. M.; Yu, W. H.; Karim, M. M.; Brannan, J. M.; Herbert, A. S.; Wec, A.Z.; Halfmann, P. J.; Fusco, M. L.; Schendel, S. L.; Gangavarapu, K.; Krause, T.; Qiu, X.; He, S.; Das, J.; Suscovich, T. J.; Lai, J.; Chandran, K.; Zeitlin, L.; Crowe, J. E. Jr; Lauffenburger, D.; Kawaoka, Y.; Kobinger, G. P.; Andersen, K. G.; Dye, J. M.; Saphire, E. O.*; Alter, G.* A Role for Fc Function in Therapeutic Monoclonal Antibody-Mediated Protection against Ebola Virus. Cell Host Microbe, 2018, 24, 221-233. PubMed

• Frei, J. C.; Wirchnianski, A. S.;Govero, J.; Vergnolle, O; Dowd, K. A.; Pierson, T. C.; Kielian, M; Girvin M. E.; Diamond, M. S.; Lai, J. R.* Engineered Dengue Virus Domain III Proteins Elicit Cross-Neutralizing Antibody Response in Mice. J. Virol. 2018, 92, pii: e01023-18. PubMed

• Nyakatura, E. K., Zak, S. E., Wec, A. Z., Hofmann, D., Shulenin, S., Bakken, R. R., Aman, M. J., Chandran, K., Dye, J. M., Lai, J. R.* Design and Evaluation of Bi- and Trispecific Antibodies Targeting Multiple Filovirus Glycoproteins. J. Biol. Chem. 2018, 293, 6201-6211. PubMed

• Lin, T. Y.; Lai, J. R.* Interrogation of Side Chain Biases for Oligomannose Recognition in Antibody 2G12 via Structure-Guided Phage Display Libraries. Bioorg. Med. Chem, 2017, 25, 5790-5798. PubMed

• Hofmann, D. H.; Zak, S. E.; Nyakatura, E. K.; Mittler, E.; Bakken, R. R.; Chandran, K.; Dye, J. M.; Lai, J. R.* Mechanistic and Fc Requirements for Inhibition of Sudan Virus Entry and In Vivo Protection by a Synthetic Antibody. Immunol. Lett., 2017, 190, 289-295. PubMed

• Wec, A. Z.; Herbert, A. S.; Murin, C. D.; Nyakatura, E. K.; Abelson, D. M.; Fels, J. M.; He, S.; James, R. M.; de La Vega, M. A.; Zhu, W.; Bakken, R. R.; Goodwin, E.; Turner, H. L.; Jangra, R. K.; Zeitlin, L.; Qiu, X.; Lai, J. R.; Walker, L. M.; Ward, A. B.; Dye, J. M.*; Chandran, K.*; Bornholdt, Z. A.* Antibodies from a Human Survivor Define Sites of Vulnerability for Broad Protection against Ebolaviruses. Cell, 2017, 169, 878-890. PubMed

• Hofmann, D.; Lai, J. R.* Exploring Human Antimicrobial Antibody Responses on a Single B Cell Level. Clin. Vaccine Immunol., 2017, 24, pii: e00544-16. PubMed

• Nyakatura, E. K.; Soare, A. Y.; Lai, J. R.* Bispecific Antibodies for Viral Immunotherapy. Hum. Vaccin. Immunother., 2017, 13, 836-842. PubMed

• Wec, A.Z.; Nyakatura, E. K.; Herbert, A. S.; Howell, K. A.; Holtsberg, F. W.; Bakken, R. R.; Mittler, E.; Christin, J. R.; Shulenin, S.; Jangra, R. K.; Bharrhan, S.; Kuehne, A. I.; Bornholdt, Z. A.; Flyak, A. I.; Saphire, E. O.; Crowe, J. E. Jr*, Aman, M. J.*, Dye, J. M.*, Lai, J. R.*, Chandran K.* A "Trojan horse" bispecific antibody strategy for broad protection against ebolaviruses. Science, 2016, 354, 350-354. PubMed

• Frei, J. C.; Lai, J. R.* Protein and Antibody Engineering by Phage Display. Methods Enzymol., 2016, 580, 45-87. PubMed

• Howell, K. A.; Qiu, X.; Brannan, J. M.; Bryan, C.; Davidson, E.; Holtsberg, F. W.; Wec, A. Z.; Shulenin, A.; Biggins, J. E.; Douglas, R.; Enterlin, S. G.; Turner, H. L.; Pallesen, J.; Murin, C. D.; He, S.; Kroeker, A.; Vu, H.; Herbert, A. S.; Fusco, M. L.; Nyakatura, E. K.; Lai, J. R.; Keck, Z.-Y.; Foung, S. K. H.; Saphire, E. O.; Zeitlin, L.; Ward, A. B.; Chandran, K.; Doranz, B.; Kobinger, G. P.; Dye, J. M.; Aman, M. J.* Antibody Treatment of Ebola and Sudan Virus Infection via a Uniquely Exposed Epitope within the Glycoprotein Receptor-Binding Site. Cell Rep. 2016, 15, 1514-1526. PubMed

• Frei, J. C.; Nyakatura, E. K.; Zak, S. E.; Bakken, R. A.; Chandran, K.; Dye, J. M.*; Lai, J. R.* Bispecific Antibody Affords Complete Post-Exposure Protection of Mice from Both Ebola (Zaire) and Sudan Viruses. Sci. Rep., 2016, 6, 19193. PubMed

• Uchime, O.; Dai, Z.; Biris, N.; Lee, D.; Sidhu, S. S.; Li, S.; Lai, J. R.*; Gavathiotis, E.* Synthetic Antibodies Inhibit Bcl-2-associated X Protein (BAX) through Blockade of the N-terminal Activation Site. J. Biol. Chem., 2016, 291, 89-102. PubMed

2011 – 2015

• Holtsberg, F. W.; Shulenin, S.; Vu, H.; Howell, K. A.; Patel, S.; Gunn, B.; Karim, M.; Lai, J. R.; Frei, J. C.;Nyakatura, E.; Zeitlin, L.; Douglas, R.; Fusco, M. L.; Saphire, E. O.; Froude, J. W.; Herbert, A. S.; Wirchnianski, A. S.; Lear-Rooney, C. M.; Alter, G.; Dye, J. M.; Glass, P. J.; Warfield, K. L.; Aman, M. J.* Pan-ebolavirus Mouse Monoclonal Antibodies: Protection against Ebola and Sudan Viruses. J. Virol., 2015, 90, 266-278. PubMed

• Frei, J. C.; Kielian, M.; Lai, J. R.* Comprehensive Mapping of Functional Epitopes on Dengue Virus Glycoprotein E DIII for Binding to Broadly Neutralizing Antibodies 4E11 and 4E5A by Phage Display. Virology, 2015, 485, 371-382. PubMed

• Liu, N.; Tao, Y.; Brenowitz, M. D.; Girvin, M. E.; Lai, J. R.* Structural and Functional Studies on the Marburg Virus GP2 Fusion Loop. J. Infect. Dis., 2015, 212 Suppl 2: S146-153. PubMed

• Malashkevich, V. N.; Higgins, C. D.; Almo, S. C.*; Lai, J. R.* A Switch from Parallel to Antiparallel Strand Orientation in a Coiled-Coil X-Ray Structure via Two Core Hydrophobic Mutations. Biopolymers: Peptide Science 2015, 104, 178-185. PubMed

• Dai, Z.; Tao, Y.; Liu, N.; Brenowitz, M. D.; Girvin, M. E.; Lai, J. R.* Conditional Trimerization and Lytic Activity of HIV-1 gp41 Variants Containing the Membrane-Associated Segments. Biochemistry 2015, 54, 1589-1599. PubMed

• Nyakatura, E. K.; Frei, J. C.; Lai, J. R.* Chemical and Structural Aspects of Ebola Virus Entry Inhibitors. ACS Infect. Dis. 2015, 1, 42-52. PubMed

• Chen, G.; Koellhoffer, J. F.; Zak, S. E.; Frei, J. C.; Liu, N.; Long, H.; Ye, W.; Nagar, K.; Pan, G.; Chandran, K.; Dye, J. M.*; Sidhu, S. S.*; Lai, J. R.* Synthetic Antibodies with a Human Framework that Protect Mice from Lethal Sudan Ebolavirus Challenge. ACS Chem. Biol. 2014, 9, 2263-2273. PubMed

• Higgins, C. D.; Malashkevich, V. N.; Almo, S. C.; Lai, J. R.* Influence of a Heptad Repeat Stutter on the pH-Dependent Conformational Behavior of the Central Coiled-Coil from Influenza Hemagglutinin HA2. Proteins. 2014, 82, 2220-2228. PubMed

• Koellhoffer, J. F.; Dai, Z.; Malashkevich, V. N.; Stenglein, M. D.; Liu, Y.; Toro, R.; Harrison, J. S.; Chandran, K.; DeRisi, J. L.; Almo, S. C.; Lai, J. R.* Structural Characterization of the Glycoprotein GP2 Core Domain from the CAS Virus, a Novel Arenavirus-like Species. J. Mol. Biol., 2014, 426, 1452-1468. PubMed

• Koellhoffer, J. F.; Higgins, C. D.; Lai, J. R.* Protein Engineering Strategies for the Development of Viral Vaccines and Immunotherapeutics. FEBS Lett., 2014, 588, 298-307. PubMed

• Liu, Y.; Higgins, C. D.; Overstreet, C. M.; Rai, K. R.; Chiorazzi, N.*; Lai, J. R.* Peptides that Bind Specifically to an Antibody form a Chronic Lymphocytic Leukemia Clone Expressing Unmutated Immunoglobulin Variable Region Genes. Mol. Med., 2013, 19, 245-252. PubMed

• Higgins, C. D.; Koellhoffer, J. F.; Chandran, K.; Lai, J. R.* C-Peptide Inhibitors of Ebola Virus Glycoprotein-Mediated Cell Entry: Effects of Conjugation to Cholesterol and Side Chain-Side Chain Crosslinking Bioorg. Med. Chem. Lett., 2013, 23, 5356-5360. PubMed

• Harrison, J. S.*; Higgins, C. D.; O’Meara, M. J.; Koellhoffer, J. F.; Kuhlman, B. A.; Lai, J. R.* Role of Electrostatic Repulsion in Controlling pH-Dependent Conformational Changes of Viral Fusion Proteins Structure, 2013, 21, 1085-1096. PubMed

• Regula, L. K.; Harris, R.; Wang, F.; Higgins, C. D.; Koellhoffer, J. F.; Zhao, Y.; Chandran, K.; Gao, J.; Girvin, M. E.; Lai, J. R.* Conformational Behavior of Peptides Corresponding to the Ebolavirus GP2 Membrane-Proximal External Region in the Presence of Micelle-Forming Surfactants and Lipids. Biochemistry, 2013, 52, 3393-3404. PubMed

• Stewart, A.; Harrison, J. S.; Regula, L. K.; Lai, J. R.* Side Chain Requirements for Affinity and Specificity in D5, an HIV-1 Antibody Derived from the VH1-69 Germline Segment. BMC Biochemistry, 2013, 14, 9. PubMed

• Koellhoffer, J. K.; Chen, G.; Sandesara, R.G.; Bale, S.; Saphire, E. O.; Chandran, K.*; Sidhu, S. S.*; Lai, J. R.* Two Synthetic Antibodies that Recognize and Neutralize Distinct Proteolytic Forms of the Ebola Virus Glycoprotein. ChemBioChem, 2012, 13, 2549–2557. PubMed

• Koellhoffer, J. K.; Malashkevich, V. N.; Harrison, J. S.; Toro, R.; Bhosle, R. C.; Chandran, K.; Almo, S. C.; Lai, J. R.* Crystal Structure of the Marburg Virus GP2 Core Domain in its Post-Fusion Conformation. Biochemistry, 2012, 51, 7665–7675. PubMed

• Harrison, J. S.; Koellhoffer, J. K.; Chandran, K.; Lai, J. R.* Marburg Virus Glycoprotein GP2: pH-Dependent Stability of the Ectodomain a-Helical Bundle. Biochemistry, 2012, 51, 2515–2525. PubMed

• Stewart, A.; Liu, Y.; Lai, J. R.* A Strategy for Phage Display Selection of Functional Domain-Exchanged Immunoglobulin Scaffolds with High Affinity for Glycan Targets. J. Immunol. Methods, 2012, 376, 150–155. PubMed

• Liu, Y.; Regula, L. K.; Stewart, A.; Lai, J. R.* Synthetic Fab Fragments that Bind the HIV-1 Heptad Repeat Regions. Biochem. Biophys. Res. Commun., 2011, 413, 611–615. PubMed

• Harrison, J. S.; Higgins, C. D.; Chandran, K.; Lai, J. R.* Designed Protein Mimics of the Ebola Virus Glycoprotein GP2 α-Helical Bundle: Stability and pH Effects. Protein Sci., 2011, 20, 1587–1596. PubMed

• Miller, E. H.; Harrison, J. S.; Radoshitzky, S. R.; Higgins, C. D.; Chi, X.; Dong, L.; Kuhn, J. H.; Bavari, S.; Lai, J. R.*; Chandran, K.* Inhibition of Ebola Virus Entry by a C-Peptide Targeted to Endosomes. J. Biol. Chem., 2011, 286, 15854–15861. PubMed

2010

• Da Silva, G. F.; Harrison, J. S.; Lai, J. R.* Contribution of Light Chain Residues to High Affinity Binding in an HIV-1 Antibody Explored by Combinatorial Scanning Mutagenesis. Biochemistry, 2010, 49, 5464–5472. PubMed